mRNA expression profile of cytokines in rat primary alveolar macrophages treated with multiwalled carbon nanotube (MWCNT)

Multiwalled carbon nanotubes (MWCNT) are fiber-shaped nanomaterials that have a potential risk for cancer due to properties similar asbestos. One type of nanotube called MWCNT-7 was categorized in Group 2B as possibly carcinogenic humans by the International Agency Research on Cancer. MWCNT-N, which is MWCNT-7, lung and pleura when administered rats via respiratory tract using intra-tracheal intra?pulmonary spraying. Macrophages an important role MWCNT induced pulmonary carcinogenicity. In this study, rat primary alveolar macrophages were employed examine possible mechanism effects MWCNT-N. MWCNT-N fractionated into flow-through retained fraction passing it through sieve with pore size 25 ?m. Microarray analysis showed up-regulation various cytokines exposed strongly expression such interleukin-6 (IL-6) macrophages. contrast, few prominently down-regulated treated The fractions did not significant effect mRNA These results provide useful information understanding MWCNT-induced carcinogenicity cytokine